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Thread | Thread Starter | Forum | Replies | Last Post |
What's the effect of large difference of library size by RNA-seq ? | yeyeming | RNA Sequencing | 8 | 03-15-2013 06:33 AM |
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Normalization of small RNA data for library size | floydian_slip | Bioinformatics | 0 | 07-10-2012 01:44 PM |
RNA-seq libraries - double peak after size selection | MLog | Sample Prep / Library Generation | 23 | 03-08-2012 11:22 AM |
Broad size range for Illumina RNA-seq library - secondary subsampling biases? | JHess | Sample Prep / Library Generation | 3 | 10-05-2011 08:38 AM |
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#1 |
Member
Location: asia Join Date: Jul 2012
Posts: 38
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Hi, folks
I am working hard to practise the RNA-seq data processing with Galaxy. What confused me a lot is that the raw data of the similar size could give very different accepted hits files in terms of size. What's wrong? For example one RNA-seq raw data is 4.2G and the accepted hits file is only 8M the other is 3.9G and the accepted hits file is bigger than 800M! The data is from from the same cell line but not the same experiment. Anyone has any idea about this? ![]() |
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