Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
What's the effect of large difference of library size by RNA-seq ? yeyeming RNA Sequencing 8 03-15-2013 06:33 AM
Public RAW RNA-seq data Now What!! prussiap Bioinformatics 13 10-03-2012 01:26 AM
Normalization of small RNA data for library size floydian_slip Bioinformatics 0 07-10-2012 01:44 PM
RNA-seq libraries - double peak after size selection MLog Sample Prep / Library Generation 23 03-08-2012 11:22 AM
Broad size range for Illumina RNA-seq library - secondary subsampling biases? JHess Sample Prep / Library Generation 3 10-05-2011 08:38 AM

Thread Tools
Old 07-20-2012, 12:23 AM   #1
Location: asia

Join Date: Jul 2012
Posts: 38
Default Why the raw RNA-seq data of the same size gave accepted hits in very different size

Hi, folks

I am working hard to practise the RNA-seq data processing with Galaxy.
What confused me a lot is that the raw data of the similar size could give very different accepted hits files in terms of size. What's wrong?
For example one RNA-seq raw data is 4.2G and the accepted hits file is only 8M
the other is 3.9G and the accepted hits file is bigger than 800M!
The data is from from the same cell line but not the same experiment.

Anyone has any idea about this?
HSV-1 is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 05:24 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO