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Old 10-14-2014, 06:39 PM   #1
Senior Member
Location: Monash University, Melbourne, Australia.

Join Date: Jan 2008
Posts: 246
Default Do you need to learn or refresh your molecular biology theory and lab bench skills?

The Micromon Recombinant DNA Techniques Course is running again this year, as it has for over 25 years. Have you been out of the lab for a while? Have a scientific background, but not that familiar with molecular biology? Want to learn some of the basic techniques and theory? Join us for this week-long intensive training course.

Hands-On Lab-Based Excercises:

The following applications are covered in the practical laboratory work spanning 30 hours of the workshop.
  • Cleavage and Ligation of Plasmid DNA
  • Transformation of Bacterial Cells
  • Preparation and Purification of Plasmid DNA
  • Restriction Enzyme Mapping
  • Agarose Gel Electrophoresis
  • Recovery of DNA from Agarose Gels
  • Preparation of Probes
  • DIG-based Southern Blotting and Hybridisation
  • Induction of Fusion Protein Production
  • Polyacrylamide Gel Electrophoresis and Western Blotting
  • Nucleic Acid Extraction
  • Reverse Transcription and PCR
  • Quantification of DNA and RNA by Bioanalysis
  • Real-time PCR (qPCR)
  • DNA Sequencing and Computer-aided Analysis
Lecture Series:

The following broad topics are covered in a series of 10 lectures throughout the week
  • Introduction to Microbiology, DNA and Molecular Biology,
  • Basic Cloning Requirements
  • Gene Cloning Techniques
  • Molecular Hybridisation (Northern, Southern and Western)
  • Construction and screening of Genomic and cDNA Libraries
  • Restriction Fragment Mapping
  • Design and use of Oligonucleotide Primers
  • Genetic Mapping
  • DNA Sequencing
  • Polymerase Chain Reaction and Real-Time PCR (qPCR)
  • Site-directed Mutagenesis
  • Cloning Vectors and their Applications
  • Gene Expression Systems
  • Basic Bioinformatics and Database Searching

Sunday 30 November Friday 5 December, 2014
Venue: Microbiology Department, Monash University (Clayton), Australia.
Fee: AU$1900 (20% off for postgrad students) - lunch, morning tea and afternoon tea are included. 20% discount for postgraduate students

Visit our website for additional information and registration

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