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Old 06-07-2018, 07:41 AM   #1
strepthroat
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Location: Cambridgeshire

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Default Comparing MiSeq output from different runs

Hi, I have about 4 different MiSeq runs i have data for. I have pipelined the fastq files through bowtie-samtools-cuffdiff to compare cDNA libraries of different samples. A thought that just occured to me is some of the times i loaded a 15pM library and sometimes i loaded a 8pM library into the MiSeq cartridge (150bp v3) .

Does anyone know if its still possible to combine the data. Does the data get normalized against total number of reads?

Thanks, Will
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Old 06-07-2018, 09:41 AM   #2
GenoMax
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If you are using DESeq2/edgeR then they will account for differences in the total number of reads. You could downsample the data to similar levels as an option.
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