Tweet
I am using Ion Torrent 200bp sequencing and looking into aligners. Any opinions as to which of the two (bowtie, bowtie2) may be a better choice? Thanks 
.
.
-
-
Bowtie cannot tolerate indels, so it should never be used for Ion Torrent alignment. So between those two, Bowtie2 is the only viable choice, though personally I'd recommend BBMap. The best aligner may depend on your experiment type and organism of interest. -
Thank you very much.... I will look into BBMap as I am not familiar with that.
Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment