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Old 09-22-2011, 03:40 AM   #1
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Default ChIP-Seq: ChIP-Seq: technical considerations for obtaining high-quality data.

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ChIP-Seq: technical considerations for obtaining high-quality data.

Nat Immunol. 2011;12(10):918-22

Authors: Kidder BL, Hu G, Zhao K

Abstract
Chromatin immunoprecipitation followed by next-generation sequencing analysis (ChIP-Seq) is a powerful method with which to investigate the genome-wide distribution of chromatin-binding proteins and histone modifications in any genome with a known sequence. The application of this technique to a variety of developmental and differentiation systems has provided global views of the cis-regulatory elements, transcription factor function and epigenetic processes involved in the control of gene transcription. Here we describe several technical aspects of the ChIP-Seq assay that diminish bias and background noise and allow the consistent generation of high-quality data.


PMID: 21934668 [PubMed - in process]



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