Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-Seq: PrimerBank: a PCR primer database for quantitative gene expression analysis, Newsbot! Literature Watch 0 11-17-2011 04:30 AM
CNV between twins... milesgr General 9 05-31-2011 11:33 AM
how to study differential expression? beliefbio Bioinformatics 23 08-05-2010 05:04 AM
PubMed: RNA-Seq - Quantitative measurement of expression through massively parallel R Newsbot! Literature Watch 0 04-02-2009 06:00 AM
In Sequence: Researchers Use SOLiD to Study Gene Expression in Single Mouse ES Cells Newsbot! SOLiD 0 11-04-2008 02:00 PM

Thread Tools
Old 02-03-2012, 08:08 PM   #1
Junior Member
Location: Australia

Join Date: Feb 2012
Posts: 1
Default quantitative expression study in twins

Hi all,

I am about to undertake a study investigating the gene expression differences between human twins using tissue from the pregnancy. I aim to quantify expression differences and hopefully allele-specific differences and splice variants between the individuals.

The abundance of transcripts differentially expressed is largely unknown and I was hoping for some input on calculating what sequencing depth I should be aiming for.

From trolling the literature I figure 8 gigabases of data per sample from 100bp paired-end Illumina Hi-seq run should give me more than enough data.

I also plan on running a 35bp single end library in addition to this for each sample for miRNA, etc.

Any thoughts, comments etc on how deep I should aim with the human transcriptome?
Sambucas is offline   Reply With Quote

depth of coverage, rna-seq

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:14 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO