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Old 02-13-2012, 08:09 AM   #1
andreitudor
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Question Illumina insertions deletions

Hello,

Is it safe to assume that a higher coverage tends to resolve the nt-substitutions that can occur in Illumina sequencing?
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Old 02-14-2012, 12:21 AM   #2
arvid
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Yes, as long as the higher coverage comes from higher input of source material the errors should be mainly random, and suppressed by more data. If you raise the coverage by amplifying your sample with more PCR cycles, then you risk to introduce more substitutions the higher "coverage" you have.
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Old 02-14-2012, 07:20 AM   #3
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Thank you. Do you know any articles that discuss this subject? Illumina substitutions?
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