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Old 12-20-2010, 04:51 AM   #1
Stephanbio
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Location: Netherlands

Join Date: Dec 2010
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Default How many mutations in WTSS

I'm a novel student bioinformatics and I am working on a transcriptome dataset of 4 cancer patients.

I've read a lot of articles about RNA-Seq. But none of the articles explains how many mutations you typically find in Rna sequencing of humans.
I have a list of 5.000 mutations that occur in all the 4 patients at same position. (and more that only occurs in 1 or 2 patients) they spand 2000 known genes.
dataset is alreay filtered for know Snp's
Isn't that too much?? How many would you typically find ? Globally ?
can't see the wood for trees

Last edited by Stephanbio; 12-20-2010 at 05:17 AM.
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Old 12-20-2010, 06:50 AM   #2
RockChalkJayhawk
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That all depends on the pipeline you used. What is your protocol for generating this SNP list?
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