Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Undercluster on MiniSeq system

    Hello everyone,

    As the title, I ran a sequencing reaction last week on MiniSeq system by Mid-Output kit 300 cycles. I determined the concentrations of library by BioAnalyzer HS chip and diluted the pooled library to 1.2 pM. When the reaction completed, the system notified me that it was undercluster (only 141k/mm2). Two points make me confused that: (1) I have optimized the concentration of library to 1.2 pM already and it was good for other runs;(2) Illumina said that the library should be diluted to 1.1 pM if I use BioAnlyzer as a quantification methods.

    I hope that someone met the similar problem with me and give me some suggestions.
    Nam Tran-huynh-bao

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 06:37 PM
0 responses
7 views
0 likes
Last Post seqadmin  
Started by seqadmin, Today, 06:07 PM
0 responses
7 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
49 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
66 views
0 likes
Last Post seqadmin  
Working...
X