Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with polypropylene for it to have a negative impact on DNA extractions?
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I have done a few large volume CTAB-Chloroform DNA extractions in polypropylene without any noticeable issues with the nucleic acids. I was told by someone with experience in a forensics lab that long-term storage of Chloroform in polypropylene will degrade the PP and may therefore result in complications. I think that you are generally fine storing chloroform in PP for a day or maybe two.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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