New instrument, HiSeq2000, first run. Overshot the recommended cluster densities on a few of the lanes. Unexpectedly the "penalty" for doing so was fairly minor:
Compared with a HiscanSQ run from last September:
Anyone know what the difference in performance is caused by?
Software is a possibility. The HiSeq is running the newest stable software -- 1.5.15, I think. Not sure about the HiScan run -- but it was about 1/2 a year ago.
Use of a control lane is a possibility. We did not use one on the HiScanSQ run.
Of course they are different instruments, and different instrument types. Although the HiScanSQ uses the same reagents and flowcells (v3) as the HiSeq.
Anyone know, or have an opinion?
--
Phillip
Compared with a HiscanSQ run from last September:
Anyone know what the difference in performance is caused by?
Software is a possibility. The HiSeq is running the newest stable software -- 1.5.15, I think. Not sure about the HiScan run -- but it was about 1/2 a year ago.
Use of a control lane is a possibility. We did not use one on the HiScanSQ run.
Of course they are different instruments, and different instrument types. Although the HiScanSQ uses the same reagents and flowcells (v3) as the HiSeq.
Anyone know, or have an opinion?
--
Phillip
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