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  • Allele specific alignemnt and quantification

    Dear all, thank you for this keeping this forum alive, it feels great,
    I have the following questions regarding allele specific alignemnt and the donwstream processing
    First how do you construct the two genomes? lets say you want to have the genome of two mouse strains, will you use the genome that is assemled by sanger or will you construct them from SNPs, and if so, how do you do that
    Second, how can you quantify this in the downstream process.
    Thank you in advance,
    cheers,
    ashu

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