hi
I'n just wondering since everybody is using different options in mapping....What is most relevant thing when I'm mapping small RNAs to ref. genome - zero mismatches or one?
Isn't allowing a mismatch can place a tag align not on his real genomic location?
I want to annotate my small seqs but need an advice which option of bowtie to use?
thanks!
I'n just wondering since everybody is using different options in mapping....What is most relevant thing when I'm mapping small RNAs to ref. genome - zero mismatches or one?
Isn't allowing a mismatch can place a tag align not on his real genomic location?
I want to annotate my small seqs but need an advice which option of bowtie to use?
thanks!