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Old 04-23-2012, 05:39 PM   #1
tmy1018
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Default Publicly available strand-specific RNA-Seq data for MAQC samples?

Hi,

Is there publicly available strand-specific RNA-Seq data for the MAQC samples?

It seems that there are people who have done this (http://www.nugeninc.com/nugen/index....brary-systems/), but I don't know if there are publicly available data that I can download.

Thanks!
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Old 06-11-2012, 09:42 AM   #2
NuGEN
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Default Strand-specific RNA-Seq data

As you indicate in the link to the NuGEN product web page we have generated strand-specific RNA-Seq data using our Encore Complete RNA-Seq Library System. We do make this data available upon request, so please contact me if you are interested in receipt of this data.

Regards,
Steve Kain
skain@nugeninc.com
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Old 06-11-2012, 07:47 PM   #3
tmy1018
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Hi Steve,

I'm more interested in using paired-end reads of the samples, do you have them available, too?

Thanks!
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Old 06-11-2012, 10:12 PM   #4
golharam
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I believe Illumina's Human Body Map data is stranded. Have you tried searching NCBI's SRA?
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Old 06-11-2012, 10:18 PM   #5
tmy1018
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Hi golharam,

I'm currently working on a method for RNA-Seq data analysis, so I'd like to use the MAQC samples where I can verify my method easily with qRT-PCR or microarray data.
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