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This is an RNA pico chip and the well is supposed to be empty.
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- Gina -
If wells before it are overloaded, it will show up in random wells. Kind of like the capillary sequencers-if one cap is high flourescence and the neighboring shows nothing, the other sequence may appear to be in that cap -
Hm. That could be it. It says the previous wells are around 200 pg/uL, so I'm not sure, unless some of that sample didn't even get measured and just went into the empty wells. Would it be bunched together in a peak that is >4000 nt like that?- GinaComment
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It can be. When it goes through the capillaries of the chip, it can let go at any time to come out at any size.Comment
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Ah I see. Thanks!- GinaComment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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