Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Size of human transcriptome/exome for coverage calculation

    Hi Guys

    This might seem like a very trivial question but strangely enough I am not able to come up with an acceptable answer.

    I am trying to calculate the size of human exome and human transcriptome in #bases for coverage purposes.

    Here is what I did

    Downloaded mRNA, exons, refSeq genes BED file from UCSC and summed up the total number of bases in each of those files / feature. Clearly there are overlapping regions in each of these annotation files but the #base that I am getting is far from the numbers one would accept. here is what I am seeing.

    1. Total #bases in mRNA : 14,881,824,369
    2. Total #bases in exons : 99,752,470
    3. Total # bases in RefSeq Genes : 2,011,862,672

    Just wondering if I should count the bases common to two genes twice or only uniq regions should be counted.

    Any pointers from your experience will help.

    Thanks!
    -Abhi

  • #2
    Hi Abhi,

    I'm not sure what other people do, but we count an exon base only once (regardless of the number of transcripts it appears in, and regardless of whether it is in an exon on one or both strands).

    We're using the UCSC Known Gene human annotation (hg19), and these are the counts we've come up with:

    Total Bases Exon Bases
    chr1 249250621 8079409
    chr2 243199373 5781424
    chr3 198022430 4706998
    chr4 191154276 3364332
    chr5 180915260 3820351
    chr6 171115067 4241245
    chr7 159138663 4049692
    chr8 146364022 2909471
    chr9 141213431 3430407
    chr10 135534747 3398919
    chr11 135006516 4439924
    chr12 133851895 4144621
    chr13 115169878 1655075
    chr14 107349540 2665222
    chr15 102531392 2897969
    chr16 90354753 3248662
    chr17 81195210 4348983
    chr18 78077248 1377184
    chr19 59128983 4500567
    chr20 63025520 2034342
    chr21 48129895 888164
    chr22 51304566 1888002
    chrX 155270560 2951340
    chrY 59373566 271506
    chrM 16571 11925

    Comment


    • #3
      Originally posted by apratap View Post
      Clearly there are overlapping regions in each of these annotation files [...] Just wondering if I should count the bases common to two genes twice or only uniq regions should be counted.
      Most of the transcribed nucleotides of the human genome are represented in different transcripts (whatever they are considered as same "gene" or not). As Bio.X2Y pointed out, you definitely have to remove redundancy. You can send your annotations to galaxy or use BEDtools to "collapse" ("project"/"fusion"/"merge") your annotated exons before adding the lengths.

      Comment


      • #4
        If you just want a base pair count for different annotations, you can just use UCSC table browser, choose the genome build you are using and annotation you are interested in, and press "summary/statistics" at the bottom, eg for hg18 RefSeq you get

        item count 34,702
        item bases 1,166,592,699 (40.49%)
        item total 2,020,112,601 (70.11%)
        smallest item 33
        average item 58,213
        biggest item 2,304,634
        block count 347,347
        block bases 66,601,430 (2.31%)
        block total 104,526,351 (3.63%)
        smallest block 3
        average block 301
        biggest block 59,461


        The "block" lines are what you are interested in: 347,347 exons from 34,702 Refseq genes, with total size of 104MB, however when removing redundancies 66Mb is covered

        Comment


        • #5
          Thanks Guys. I understand that it is acceptable to remove redundancy at exon level.

          @frozenlyse : your end number (exons) seems to match mine.

          How do I deal with gene level coverage. There are many genes which overlap each other and as noted in my first post.

          Total # bases in RefSeq Genes : 2,011,862,672

          Is it acceptable to remove redundancy while counting bases in all human genes. In a way this will lead us to underestimate coverage. I say so because overlapping genes can be coexpressed right >>?

          Thanks for your time to help me understand this.

          Best,
          -Abhi

          Comment


          • #6
            I assume you are interested in this since you are doing whole exome sequence enrichment and subsequent sequencing.

            Different vendors have different amounts of "whole exome" coverage. We found that the Agilent Sure Select only enriches for ~89% of the human whole exome.

            Comment


            • #7
              Originally posted by NextGenSeq View Post
              I assume you are interested in this since you are doing whole exome sequence enrichment and subsequent sequencing.

              Different vendors have different amounts of "whole exome" coverage. We found that the Agilent Sure Select only enriches for ~89% of the human whole exome.
              NextGenSeq, how did you get the number of ~89% exome targetted by agilent? Could you share some detail on that!

              Thanks,
              sm
              --
              bioinfosm

              Comment


              • #8
                By comparing the genes listed in the bed file to the UCSC annotation. I tried attaching the bed file but it's too large for this site to allow it.

                Comment


                • #9
                  I keep seeing a figure of 30-33Mb for the human exome e.g.

                  This 2009 Nature paper

                  "Protein-coding regions constitute ~1% of the human genome or ~30 megabases (Mb), split across ~180,000 exons."

                  30-33Mb is also the figure cited in Illumina's "Sequencing Output Calculator' , sent to me by tech support.

                  Anyone know why the number is so much higher on this thread?

                  Comment


                  • #10
                    I would also like to know why the huge discrepancy between what's in the literature (~30-40Mb) and the numbers cited in this thread. I just checked the GENCODE v6 annotations and the total annotated base count is over a billion, supporting the estimates in this thread. I'm confused. Can anyone clear up the discrepancy?

                    Comment


                    • #11
                      Our friend Mr. Ref Seq says ...

                      Back of the envelope calculations:
                      The sum of the values for base coverage of the exons for the data above in the hg19/UCSCknown table (posted above) is
                      81,105,734

                      The Refseq table from UCSC for hg19 (jan 2011 version) says : 63,995,498
                      [ method : load table into datastruct, sort by name, traverse, if (currentname==previousname) dont count else calculate sum of exons and add to sum]. Notabene: this won't eliminate some overlapping situations.

                      Refseq is more conservative than UCSCknown and relies more on hand curation and less on computation.

                      I don't know about GENCODE but if it's that for human only and that number is right then it's probably any transcript ever measured. I could only speculate on what that extra bonus coverage is. A free trip to Sweden goes to the guy that can explain and prove it (if it's functionally real).

                      Comment


                      • #12
                        Just to throw my 2 Cents in. As far as I know most exome-enriching kits use the CDS database for generating the exome library. As this database is less comprehensive than the Refseq or knownGene annotations in UCSC some exons will be missed due to that. Of course others are discarded because of hybridization difficulties (repetitive regions, etc).

                        Comment


                        • #13
                          Originally posted by ssully View Post
                          I keep seeing a figure of 30-33Mb for the human exome e.g.

                          This 2009 Nature paper

                          "Protein-coding regions constitute ~1% of the human genome or ~30 megabases (Mb), split across ~180,000 exons."

                          30-33Mb is also the figure cited in Illumina's "Sequencing Output Calculator' , sent to me by tech support.

                          Anyone know why the number is so much higher on this thread?
                          Because "protein coding regions" and "exons" are different things. UTRs can be long, especially in human.

                          I think it is important to know what we are talking about:

                          1. number of genomic positions that are annotated as coding (included in CDS)
                          2. number of genomic positions that are annotated as exonic (included in exons)

                          As frozenlyse and Richard Finney indicated, values for 2. range around 60 and 80Mb, depending on the annotation source.
                          Ssully, the citation you mention with the number of 30Mb refers to 1. ("protein coding regions").
                          Rstarke, what is this number of 1 billion referring to? "Annotated bases" can be anything, on a genome you can annotate introns, promoters, repeated regions.. a link to this information would help.
                          Now, is there a precise definition of "exome" or is it a loose term? Is it supposed to include coding regions only, or can anyone put in there some UTR, promoters, intronic flanks, etc?

                          Comment


                          • #14
                            Originally posted by ulz_peter View Post
                            Just to throw my 2 Cents in. As far as I know most exome-enriching kits use the CDS database for generating the exome library. As this database is less comprehensive than the Refseq or knownGene annotations in UCSC some exons will be missed due to that. Of course others are discarded because of hybridization difficulties (repetitive regions, etc).
                            That makes sense, thanks.

                            Comment


                            • #15
                              Sorry to revive this thread, but exons or CDSs are not the only thing transcribed, so how can we account for non-coding RNAs? My understanding is that they are also part of the transcriptome...

                              Comment

                              Latest Articles

                              Collapse

                              • seqadmin
                                Strategies for Sequencing Challenging Samples
                                by seqadmin


                                Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                                03-22-2024, 06:39 AM
                              • seqadmin
                                Techniques and Challenges in Conservation Genomics
                                by seqadmin



                                The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

                                Avian Conservation
                                Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
                                03-08-2024, 10:41 AM

                              ad_right_rmr

                              Collapse

                              News

                              Collapse

                              Topics Statistics Last Post
                              Started by seqadmin, Yesterday, 06:37 PM
                              0 responses
                              10 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, Yesterday, 06:07 PM
                              0 responses
                              9 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, 03-22-2024, 10:03 AM
                              0 responses
                              50 views
                              0 likes
                              Last Post seqadmin  
                              Started by seqadmin, 03-21-2024, 07:32 AM
                              0 responses
                              67 views
                              0 likes
                              Last Post seqadmin  
                              Working...
                              X