Does anyone have a suggestion for an enzyme cocktail to perform the extension-ligation step similar to what is shown in the Illumina TruSeq Custom Amplicon workflow step 2? Note that probe 1 and probe 2 are in the same orientation. This is not a PCR step. It is more of a gap filling step.
Illumina will not tell me what is in the secret sauce aside from it contains a "ligase and a polymerase in proprietary formulation".
Illumina will not tell me what is in the secret sauce aside from it contains a "ligase and a polymerase in proprietary formulation".
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