Hi,
I have 6 samples with which I am trying to make a library with Illumina TruSeq Exome Library Prep Kit (starting material was 100-300 ng).
The problem is that before the exome capture, the first enrichment of my fragments does not work (fragment concentrations are in a 0.5 - 3 ng/ul range measured with PicoGreen).
Knowing that I need to pull 200 ng of fragments per sample before exome capture, I cannot even go farther.
I used these same samples to do qPCRs (KAPA) and whole genome amplifications (QIAGEN Repli-g) and it worked great.
Illumina tech support has not been very helpful so far, so I would have few questions:
1) Do you think I could use the QIAGEN Whole Genome Amplification kit I used before instead of Illumina reagents?
2) Any impact on the adaptor sequences? (I don't know what amplification method is used in the Illumina kit)
3) Does this happened to someone before? Can the kit reagents be faulty?
Thanks !
I have 6 samples with which I am trying to make a library with Illumina TruSeq Exome Library Prep Kit (starting material was 100-300 ng).
The problem is that before the exome capture, the first enrichment of my fragments does not work (fragment concentrations are in a 0.5 - 3 ng/ul range measured with PicoGreen).
Knowing that I need to pull 200 ng of fragments per sample before exome capture, I cannot even go farther.
I used these same samples to do qPCRs (KAPA) and whole genome amplifications (QIAGEN Repli-g) and it worked great.
Illumina tech support has not been very helpful so far, so I would have few questions:
1) Do you think I could use the QIAGEN Whole Genome Amplification kit I used before instead of Illumina reagents?
2) Any impact on the adaptor sequences? (I don't know what amplification method is used in the Illumina kit)
3) Does this happened to someone before? Can the kit reagents be faulty?
Thanks !
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