Hello everyone,
I just have a question about something I read on a paper(Burke et al 2010, 467, 587–590), which I dont quite understand...
This is from the methods
"Genome sequencing. DNA was extracted from 25 female flies collected from
each of the ACO1–ACO5 and CO1–CO5 populations and pooled within selection
treatments to make two Illumina paired-end libraries. We also created a library for the ACO1 replicate population only. The pooled libraries were each run on four (unpaired 54-bp) lanes of an Illumina Genome Analyser II, and the ACO1 library was run on a single (paired-end 36-bp) lane".
So the pooled samples, ACO1–ACO5 and CO1–CO5, were run using paired end... but below they say they were run in unpaired 54 bp lanes... so they were not paired end? btw paired end basically means they sequenced both strands... right?
TIA for your time!
Fred
Im completely new to these techniques, my only experience is with good old drhod sequencing. Im guessing this is obvious for most of you here, so I ask... can anyone point me to a accessible guide that explain how this Illumina NGS works?.
I just have a question about something I read on a paper(Burke et al 2010, 467, 587–590), which I dont quite understand...
This is from the methods
"Genome sequencing. DNA was extracted from 25 female flies collected from
each of the ACO1–ACO5 and CO1–CO5 populations and pooled within selection
treatments to make two Illumina paired-end libraries. We also created a library for the ACO1 replicate population only. The pooled libraries were each run on four (unpaired 54-bp) lanes of an Illumina Genome Analyser II, and the ACO1 library was run on a single (paired-end 36-bp) lane".
So the pooled samples, ACO1–ACO5 and CO1–CO5, were run using paired end... but below they say they were run in unpaired 54 bp lanes... so they were not paired end? btw paired end basically means they sequenced both strands... right?
TIA for your time!
Fred
Im completely new to these techniques, my only experience is with good old drhod sequencing. Im guessing this is obvious for most of you here, so I ask... can anyone point me to a accessible guide that explain how this Illumina NGS works?.
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