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Old 11-30-2020, 09:33 AM   #1
Junior Member
Location: Barcelona

Join Date: Nov 2011
Posts: 5
Default Nanopore library on top of an Illumina library

Hi all,
we don't have the original aliquote of an old sample, but we have an Illumina Nextera Flex library in the freezer.

I am aware that the ends are y-shaped, correct? Anyone of you have a fancy protocol to remodel/fill/modify those ends that I can do a Nanopore library on top?

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Old 11-30-2020, 09:45 AM   #2
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Location: US

Join Date: Dec 2010
Posts: 453

No remodeling needed.
All Nextera libraries are PCR amplified to be sequenceable. After the PCR amplification they are wonderfully double-stranded throughout and should be blunt-ended (if a high-fidelity polymerase was used).

BTW, Truseq-style libraries have Y-adapters; Nextera/transposase libraries work differently.
luc is offline   Reply With Quote

ends, illumina, library construction, nanopore, y-shape

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