Hi everyone,
I'm doing an annotation using RAST in a non-ordered contigs genome and I'm having trouble to determine if the annotation was done properly or not, because the reference genomes avaible are not that close to my microorganism... so I have almost half of my CDS not agrouped in subsystems, and also, I can't tell how bad it is.
How can I evaluate the quality of the annotation? How can I decide if a CD is properly annotated or not?
Can anyone help me, please ?
I'm doing an annotation using RAST in a non-ordered contigs genome and I'm having trouble to determine if the annotation was done properly or not, because the reference genomes avaible are not that close to my microorganism... so I have almost half of my CDS not agrouped in subsystems, and also, I can't tell how bad it is.
How can I evaluate the quality of the annotation? How can I decide if a CD is properly annotated or not?
Can anyone help me, please ?
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