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Old 08-01-2020, 09:35 AM   #1
NewbtoNGS
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Question Will my run fail?

I recently loaded a MiSeq that had low library yields ~1-2nM. Does this indicate my sequencing run will fail? Even though the Q30 and PF look good?

Will I get usable data from this?
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Old 08-01-2020, 04:59 PM   #2
luc
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The run is still ongoing?
More information would be rquired.
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Old 08-01-2020, 05:36 PM   #3
NewbtoNGS
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Yes. The run is still running. %PF 91%, Q30 92%.
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Old 08-02-2020, 05:18 PM   #4
luc
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I beleive there is no reason to expect the run to fail now. The data should be usable as indicated by the qualityscores.
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