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Old 01-26-2012, 08:48 AM   #1
Vartul
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Default Roche 454 RL adapters.. help!!!

Hi,

We have prepared a paired end library for 454 GS junior but now would like to try rapid library protocol on a couple of isolates. We have all the reagents needed for the rapid library preparation except for adaptors. I asked Roche but they do not supply adaptors separately . We do not want to multiplex several libraries therefore, a MID adaptor kit would be useless. It will also cost a fortune as will cost a new RL library preparation kit. We have noticed that IDT (http://eu.idtdna.com/order/seqset.aspx?type=rapid) can synthesize a set of (two) adaptors. I would request for an advice if a set of adaptors synthesized by IDT can be used instead of Roche adaptors? If yes, what should be the concentration of these oligos in the ligation reaction?
Roche supplies a single tube with Y shaped adaptors and according to their protocol, we just need to add 1ul to the reaction.
Thank you,
Vartul
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Old 01-26-2012, 02:57 PM   #2
RCJK
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Default

This technical bulletin should help answer your questions.
Attached Files
File Type: pdf TCB-10010_MIDAdaptorsforRapidLibraryPreparations.pdf (426.1 KB, 149 views)
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Old 01-27-2012, 02:39 AM   #3
Vartul
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Dear RCJK, many thanks for the technical bulletin.
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Old 09-11-2020, 08:23 AM   #4
kevin zhang
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Post please give me this technical bulletin

Quote:
Originally Posted by Vartul View Post
Dear RCJK, many thanks for the technical bulletin.
Dear sir, can you give me this technical bulletin?thank you so much.
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