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  • Problems about Bowtie

    Our bench experimental data come from Solexa, which would offer us two BP ends.
    Now, I'd like to use Bowtie to align the two end BP-sequencing with H. sapiens chromosomes.
    Then, I use the following operation command:
    ./bowtie -a -v 2 -p 16 chr21 -q -1 TestData/s_1_1_sequence.txt -2 TestData/s_1_2_sequence.txt

    I had transformed the chr21 to ebwt, which would be my chromosome sequence target.
    The two txt files are the BP end sequences.

    What I want to know is :
    1. There must be some BPs between two end sequences.
    Could I know how many BPs between two end sequences in my commands?
    2. If my current command couldn't do the alignment, how could I modify my command to get it? ( The middle sequences is 200 bp)


    Thanks a lot!

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