Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Template Switching RT Basic Q aryehhirt Bioinformatics 1 06-15-2018 06:16 PM
Multiple template switching in SmartSeq2 ericoml Sample Prep / Library Generation 0 02-26-2018 07:02 AM
RT Enzyme for template switching in SMART cDNA snotarba Sample Prep / Library Generation 1 07-09-2015 07:49 AM
PCR Jumping (Template Switching) in Amplicon sequencing eladSeq 454 Pyrosequencing 2 04-02-2012 08:10 AM

Thread Tools
Old 10-02-2018, 09:15 AM   #1
Location: Maryland

Join Date: May 2011
Posts: 65
Default Template-switching RTase preferences

Does anyone out there have strong feelings about which RTase enzymes are best for transcriptome library prep using template-switch oligos? Apparently, SmartScribe is Clontech's favorite. Others we've tried include PrimeScript, ProtoScript, Maxima, and Superscript II.

Thanks for any input!

eab is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:26 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO