Tweet
We are developing a workflow for using the Ampliseq Cancer Panel starting with DNA from single cells. We plan to use WGA but wonder if someone could recommend a kit. Genomiphi for example requires 10 ngs. A single cell will have about 6 pgs. Any advice?
-
-
The following paper has a method to purify the Phi29 polymerase away from DNA contamination, which can be a problem when amplifying low concentrations of DNA. I am not sure if there is a commercial product yet.
Blainey, P. C. and S. R. Quake (2011). "Digital MDA for enumeration of total nucleic acid contamination." Nucleic Acids Research 39(4) e19. -
Qiagen has just introduced a product that is a single cell WGA.
Don't have any experience with it but might be a good starting point.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment