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Old 07-11-2018, 10:59 AM   #1
Junior Member
Location: Turkey

Join Date: Jul 2018
Posts: 3
Default What is the process after mapping ?

Hi guys,

I have a raw data, which is rna-seq paired end. I have downloaded genome reference from ncbi. Then, I have mapped rna seq to genome reference through tophat2. I had some results like accepted.hits.bam. After this process, I used the cufflinks for assembly. Finally, I have a transcripts.gtf file. Firstly, I do not know how to interprate these files. Secondly, After all this process, What i should do ? I mean, What options do i have ?
Well, Should i use artemis or igv ? Actually i dont really know.

Thanks in advance,
your respectfully,
ugurcabuk is offline   Reply With Quote
Old 08-05-2018, 06:53 AM   #2
David Eccles (gringer)
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 835

What is the question you are trying to answer by doing this mapping?
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