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  • Error in GSMapper output, 454Allcontigs.fna and 454NewblerMetrics.txt

    Hi all,

    I mappped reads to reference using GSMapper, it generated result files. When i see 454AllContigs.fna, it shows two contigs generated, first contig is of size 1155 nt numreads used to form is 20712 reads.

    But I actually added only ~14,000 reads to map and 454NewblerMetrics.txt supporting this, so why first contig is showing, num of reads used to form is 20712 reads?

    What is difference between, Partially Mapped, Chimeric sequences?

    Thanks.
    Attached Files

  • #2
    The too large number of reads must be a bug...

    Partially mapped: part of the read could be mapped, the rest not.
    Chimeric: different parts of the read mapped to two or more discontinuous locations

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