Hello all
I want to sequence the transcriptome of a new organism (species of lizard)
i order to do that, i need first to extract rna from fresh tissue. i follow protocol but I got an rna result that suggesting that my sample was degredated. but because I don't know many people who try to extract rna from reptiles i dont know if maybe the sample suppose to be different from standard extraction from mammals.
attached please find a pdf version of bio analyzer and gel electrophoresis of my samples (samples 11 and 12)
will be happy to hear ideas
Dan
I want to sequence the transcriptome of a new organism (species of lizard)
i order to do that, i need first to extract rna from fresh tissue. i follow protocol but I got an rna result that suggesting that my sample was degredated. but because I don't know many people who try to extract rna from reptiles i dont know if maybe the sample suppose to be different from standard extraction from mammals.
attached please find a pdf version of bio analyzer and gel electrophoresis of my samples (samples 11 and 12)
will be happy to hear ideas
Dan
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