3'UTR library or random primed cDNA library for quantification?

Rosanne82 · 06-26-2009, 06:27 AM

I am quite new in the field of next generation sequencing and I hope to get some information and help here.
I want to do a RNA-Seq by using the Illumina platform to analyze the transcript profile of a specific tissue of a wildtype plant compared to a mutant. The aim is identifying a different transcription level of some genes as well as finding some rare genes. The organism I am working with is not sequenced yet, but it should be till the end of this year.
My question is which type of library is more suitable for quantification, a random primed cDNA library or a 3’UTR library, which proposed me a company. But what would be the advantage of a 3’UTR library?

Thank you very much!

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Library quantification: opinions?	krobison	Sample Prep / Library Generation	41	06-23-2016 07:38 PM
Library quantification	suludana	Illumina/Solexa	22	10-24-2013 04:52 PM
Illumina library quantification	deepakpatilp	Illumina/Solexa	75	06-24-2013 10:21 AM
RNA-seq from oligo(dT) primed cDNA	ETHANol	RNA Sequencing	1	11-23-2011 11:37 AM
Library Quantification Confusion!	peromhc	Sample Prep / Library Generation	9	10-05-2011 08:18 AM

06-26-2009, 06:27 AM	#1
Rosanne82 Junior Member Location: Germany Join Date: May 2009 Posts: 1	3'UTR library or random primed cDNA library for quantification? I am quite new in the field of next generation sequencing and I hope to get some information and help here. I want to do a RNA-Seq by using the Illumina platform to analyze the transcript profile of a specific tissue of a wildtype plant compared to a mutant. The aim is identifying a different transcription level of some genes as well as finding some rare genes. The organism I am working with is not sequenced yet, but it should be till the end of this year. My question is which type of library is more suitable for quantification, a random primed cDNA library or a 3’UTR library, which proposed me a company. But what would be the advantage of a 3’UTR library? Thank you very much!