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Old 03-19-2015, 02:06 PM   #1
Nighthawkrao77
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Location: ND

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Default Scale Up or Down Reaction Volumes?

Has anyone used TruSeq RNA or NEBNext RNA kits and tried to scale the volumes down or up? Is this even possible? Just curious to see if I can add more to generate more libraries, or less to proportionally add less RNA input.
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Old 03-19-2015, 03:28 PM   #2
kerplunk412
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Scaling up a reaction volume will not result in more library, unless you scale up the input accordingly. Pretty much all NGS kits should already get you much more library than you need for sequencing if you use the highest recommended input, so there wouldn't be much point in scaling up a reaction, in my opinion.

Scaling down a reaction could be performed, but it probably will not allow you to go below the recommended input. The biggest advantage of scaling down is saving money. Scaling down may require some slight optimization, but if you have a lot of samples to prep it may be worth the optimization if you can make a kit last twice as long.
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