Mixing cDNA samples from Ovation RNA‐Seq System V2 of NuGEN with Nextera Dual Indexes

seq_uestion · 11-18-2019, 02:04 AM

Hello,

I have a question regarding the compatibility of the "IDT® for Illumina Nextera™ DNA UD Indexes". Illumina says "These index kits are NOT interchangeable with the Nextera DNA or Nextera XT Index Kits".
But, are they compatible with amplified cDNA samples prepared using the Ovation® RNA‐Seq System V2 of NuGEN from RNA?
Our preferred sequence facility (uses HiSeq4000) has strongly recommended the use of duel index adaptors (in specific the Ovation® Ultralow System V2 developed by Illumina) to avoid index hoping, we will like to know if Nextera indexes are also an option,

Best regards

luc · 11-19-2019, 08:34 AM

Best ask Nugen. Likely Nugen is using the Truseq design for their kits, not Nextera sequences.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
NuGen Ovation Universal RNA-seq on Torrent	zara	Ion Torrent	2	01-27-2015 01:59 AM
NuGEN Ovation® RNA‑Seq with PacBio sequencing	aliceli	Sample Prep / Library Generation	0	11-28-2012 07:52 AM
New! Ovation RNA-Seq System V2 from NuGEN Technologies	NuGEN	Vendor Forum	2	07-21-2011 09:46 PM
Alternatives to Nugen Ovation RNA-Seq	pbluescript	Sample Prep / Library Generation	1	02-13-2011 02:14 PM
Experience with nugen ovation 3 dge System	moritzhess	Sample Prep / Library Generation	0	11-06-2010 02:38 PM

11-18-2019, 02:04 AM	#1
seq_uestion Junior Member Location: Germany Join Date: Nov 2019 Posts: 1	Mixing cDNA samples from Ovation RNA‐Seq System V2 of NuGEN with Nextera Dual Indexes Hello, I have a question regarding the compatibility of the "IDT® for Illumina Nextera™ DNA UD Indexes". Illumina says "These index kits are NOT interchangeable with the Nextera DNA or Nextera XT Index Kits". But, are they compatible with amplified cDNA samples prepared using the Ovation® RNA‐Seq System V2 of NuGEN from RNA? Our preferred sequence facility (uses HiSeq4000) has strongly recommended the use of duel index adaptors (in specific the Ovation® Ultralow System V2 developed by Illumina) to avoid index hoping, we will like to know if Nextera indexes are also an option, Best regards

11-19-2019, 08:34 AM	#2
luc Senior Member Location: US Join Date: Dec 2010 Posts: 422	Best ask Nugen. Likely Nugen is using the Truseq design for their kits, not Nextera sequences.