Hi all,
I am planning to do an expression study by sequencing mRNA.
The standard protocol would be following Truseq sample prep kit.
However, I read somewhere that
1. remove riboRNA by Ribo Zero
2. add poly A tail to leftover RNA
3. use poly dT for cDNA synthesis
If that's the case, is it possible to use Nextera or Nextera XT to sequence the cDNA?
I am planning to do an expression study by sequencing mRNA.
The standard protocol would be following Truseq sample prep kit.
However, I read somewhere that
1. remove riboRNA by Ribo Zero
2. add poly A tail to leftover RNA
3. use poly dT for cDNA synthesis
If that's the case, is it possible to use Nextera or Nextera XT to sequence the cDNA?
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