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Old 02-24-2010, 06:30 PM   #1
asiebel
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Location: Melbourne, Australia

Join Date: Feb 2010
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Default Minimum amount of total RNA for mRNA-seq

I have a few total RNA samples that I would like to prepare for the Illumina GA platform, however they are just below the threshold stated in the protocol for processing for mRNA-seq. They recommend 1-10ug total RNA, but I have 800ng in my sample. Has anyone validated this protocol for lower amount of starting material (total RNA)? I would like to avoid pre-amplification of my RNA if possible. Cheers!
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