SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-seq libraries - double peak after size selection MLog Sample Prep / Library Generation 23 03-08-2012 10:22 AM
PE (in gel) Size-Selection puggie Illumina/Solexa 3 11-28-2011 04:24 AM
Need help: Weird size selection chromatin Sample Prep / Library Generation 0 11-15-2010 03:24 AM
Double AMPure size selection in Titanium 3kb span paired end library H5N1 Sample Prep / Library Generation 5 05-03-2010 10:34 AM
Size Selection Tricks?? wiggy 454 Pyrosequencing 0 06-18-2009 10:11 AM

Reply
 
Thread Tools
Old 10-09-2010, 02:36 PM   #1
Bjorn
Junior Member
 
Location: US

Join Date: Sep 2010
Posts: 2
Default double AMPure size selection

Hi,

As shown in Lennon et al (2010, Genome Biol.) and Rodrigue et al (2010, Plos One), the Broad and many other folks seem to routinely employ a double AMPure bead approach to obviate the need for gels and to make size selection more scalable. For example, Lennon et al first use a 0.5 ratio and then a 0.7 ratio of beads to hone in on a size range of 300-1000bp.

I would like to try this out for Illumina prep, but I haven't found a lot of details on optimizations for desired size ranges. My goal is a quite narrow size range (400-500bp fragments), and I wonder if the double AMPure approach can do this for me.

Are there any good titration curves out there that would give me an idea of what AMPure ratios would do the job?

Thanks for any insights.

Bjorn
Bjorn is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:50 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO