I have read somewhere that PCR products can be turned into libraries using Illumina's genomic sample prep kit and then sequenced on the GAIIx. Can someone explain who would need this kind of sequencing done, and how this all works while making the library? I also read somewhere that the PCR products must be ligated together first in order for library construction and sequencing to work properly, something about the ends of the PCR products having more bias during sequencing, so if they are ligated together it ensures the entire product is read through with less bias. I am not sure if I got that right, I hope someone knows what I am trying to say Also explaining any bias that exist in this kind of library construction will be helpful.
And just to make it clearer, the starting material is PCR products that will be built into libraries, not the usual genomic DNA.
Thanks for clarifications.
And just to make it clearer, the starting material is PCR products that will be built into libraries, not the usual genomic DNA.
Thanks for clarifications.
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