Hi, I'm doing a WGS sequencing project. The original samples provided were whole human blood in EDTA. After DNA extraction and library gen with the TruSeq DNA PCR-Free protocol w/ 350 bp insert, I got the following results on Tapestation (gel and histogram of one of the 5 libraries, which looks similar to them all):
The result looks a lot different from the figures in the Illumina Library QC whitepaper, so I was wondering if anyone knows if it's a good idea to proceed to sequencing now with this result? If not, any ideas on what went wrong?
I'm concerned because I need to save project budget for the sequencing itself, so I'd rather re-do the DNA extract and library gen than sequence bad libraries and get bad data.
Thanks in advance!
The result looks a lot different from the figures in the Illumina Library QC whitepaper, so I was wondering if anyone knows if it's a good idea to proceed to sequencing now with this result? If not, any ideas on what went wrong?
I'm concerned because I need to save project budget for the sequencing itself, so I'd rather re-do the DNA extract and library gen than sequence bad libraries and get bad data.
Thanks in advance!
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