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Thread | Thread Starter | Forum | Replies | Last Post |
Single Cell RNASeq- Help | rammohanshukla | General | 0 | 09-06-2019 10:09 PM |
What to do if you only have a single RNAseq condition? | bastianwur | RNA Sequencing | 0 | 10-06-2016 08:14 AM |
Selecting Mitochondria and nuclear genome in libarary preaparation | mhkiani | Sample Prep / Library Generation | 1 | 10-22-2014 07:31 PM |
RNAseq from single cell RT? | pie | Sample Prep / Library Generation | 0 | 10-29-2010 09:41 AM |
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#1 |
Junior Member
Location: St. Louis Join Date: Jul 2015
Posts: 4
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for snRNAseq, do people use mitochondrial filtering? I'm not sure if this still makes sense even though we are working with data from nuclei rather than whole cells, but I do see some expression of mito genes in my data.
Also, is it preferable to filter the cells based on mito content or to regress it out? |
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Tags |
scrnaseq, seurat, single cell rnaseq, snrnaseq |
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