Using scRNA-seq to help with snRNA-seq clustering

Matt.Kanke · 08-11-2020, 05:38 PM

Hi,

I am working with a single nucleotide RNA-seq (snRNAseq) dataset. These are often hard to cluster compared to single cell RNA-seq (scRNAseq). I was wondering if you could combine a snRNAseq and scRNAseq using Seurat's integration method, and after assigning clusters, filter out the scRNAseq cells leaving you with (hopefully) better clustered snRNAseq data to continue the analyses.

Thanks,
Matt

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
scRNA-seq	Physalia-courses	RNA Sequencing	0	11-01-2018 05:11 AM
scRNA-Seq	seqgirl123	RNA Sequencing	2	12-12-2016 05:57 PM

08-11-2020, 05:38 PM	#1
Matt.Kanke Junior Member Location: Chapel Hill, NC Join Date: Sep 2015 Posts: 4	Using scRNA-seq to help with snRNA-seq clustering Hi, I am working with a single nucleotide RNA-seq (snRNAseq) dataset. These are often hard to cluster compared to single cell RNA-seq (scRNAseq). I was wondering if you could combine a snRNAseq and scRNAseq using Seurat's integration method, and after assigning clusters, filter out the scRNAseq cells leaving you with (hopefully) better clustered snRNAseq data to continue the analyses. Thanks, Matt