Hi all!
Though there have been many similar posts, but to have an updated (and more recent) thought, hence I am posting it again.
I have some Illumina GAIIX paired end data in two separate files (with their suffix as _1 and _2). Before putting them for assembly, I need to perform certain pre processing of these file to alleviate its quality (probably FASTQ MASKER by quality control on GLAXY web tools). My query is " Should I join these paired end data first and then go on with the quality treatments OR should I just perform the quality treatment and then join the two files.
Thanks in advance
Any help will be deeply appreciated.
Though there have been many similar posts, but to have an updated (and more recent) thought, hence I am posting it again.
I have some Illumina GAIIX paired end data in two separate files (with their suffix as _1 and _2). Before putting them for assembly, I need to perform certain pre processing of these file to alleviate its quality (probably FASTQ MASKER by quality control on GLAXY web tools). My query is " Should I join these paired end data first and then go on with the quality treatments OR should I just perform the quality treatment and then join the two files.
Thanks in advance
Any help will be deeply appreciated.
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