Go Back   SEQanswers > Applications Forums > Metagenomics

Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Massively parallel sequencing for the detection of adventitious viruses. Newsbot! Literature Watch 0 02-03-2012 12:20 AM
PubMed: Genotypic and phenotypic resistance of pandemic A/H1N1 influenza viruses circ Newsbot! Literature Watch 0 11-13-2010 03:00 AM

Thread Tools
Old 02-13-2009, 06:00 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Metagenomic analyses of viruses in the stool of children with acute flaccid p

Syndicated from PubMed RSS Feeds

Related Articles Metagenomic analyses of viruses in the stool of children with acute flaccid paralysis.

J Virol. 2009 Feb 11;

Authors: Victoria JG, Kapoor A, Li L, Blinkova O, Slikas B, Wang C, Naeem A, Zaidi S, Delwart E

We analyzed viral nucleic acids in stool samples collected from 35 South Asian children with non-polio acute flaccid paralysis (AFP). Sequence-independent reverse transcription and PCR amplification of viral capsid-protected nuclease-resistant nucleic acids was followed by DNA sequencing and sequence similarity searches to identify known and new viruses. Limited Sanger sequencing (35-240 subclones per sample) identified an average of 1.4 eukaryotic viruses per sample, while pyrosequencing yielded 2.6 viruses per sample. In addition to bacteriophage and plant viruses, we detected known enteric viruses including rotavirus, adenovirus, picobirnavirus, and human enterovirus (HEV) species A-C, as well as numerous other members of the Picornaviridae family including parechovirus, Aichi virus, rhinovirus, and human cardiovirus. The most divergent viral sequences relative to reported viruses included members of a novel Picornaviridae genus and four new viral species (members of the Dicistroviridae, Nodaviridae, Circoviridae families and the Bocavirus genus). Six healthy contacts of AFP cases were similarly analyzed and also contained numerous viruses, particularly HEV-C, including a potentially novel Enterovirus genotype. Determining the prevalence and pathogenicity of the novel genotypes, species, genera, and potential new viral families identified in this study in different demographic groups will require further studies now facilitated by knowledge of their genomes.

PMID: 19211756 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:06 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO