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Old 12-02-2013, 05:50 AM   #1
SS00
PhD Student
 
Location: La Jolla

Join Date: Jun 2012
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Default Can someone explain how Ribo Zero works?

I'm using the Ribo Zero Magnetic Kit from Epicentre and I get that somehow my rRNA ends up bounds to the beads and my ribo-depleted RNA is in the supernatant but I am not sure on the specifics. Can anyone help with this? Specifically: -

- What is in the ribosomal removal solution. Are they oligos that are specific for ribosomal RNA?

- I have found a paper mentioning that the ribo zero kit uses a hybrid substraction technique but not sure what this is?

- Why does my ribosomal RNA end up bound to beads but the rest doesn't?
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Old 12-02-2013, 06:20 AM   #2
kmcarr
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Quote:
Originally Posted by SS00 View Post
I'm using the Ribo Zero Magnetic Kit from Epicentre and I get that somehow my rRNA ends up bounds to the beads and my ribo-depleted RNA is in the supernatant but I am not sure on the specifics. Can anyone help with this? Specifically: -

- What is in the ribosomal removal solution. Are they oligos that are specific for ribosomal RNA?

- I have found a paper mentioning that the ribo zero kit uses a hybrid substraction technique but not sure what this is?

- Why does my ribosomal RNA end up bound to beads but the rest doesn't?
Check the Ribo-Zero FAQ, specifically #9 and #14, and a better description is hybridization/capture not hybrid subtraction.
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Old 12-03-2013, 02:04 AM   #3
SS00
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Originally Posted by kmcarr View Post
Check the Ribo-Zero FAQ, specifically #9 and #14, and a better description is hybridization/capture not hybrid subtraction.
Brill, thanks!
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Old 12-23-2013, 01:08 PM   #4
Phage434
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For a detailed description of protocols, see the patent application US 2011/0040081 A1
Methods, Compositions, and Kits for Generating rRNA-Depleted Samples or Isolating rRNA from Samples, Roy. R. Sooknanan, assigned to Epicentre
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