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Old 03-08-2010, 12:54 PM   #1
Location: Germany

Join Date: Feb 2010
Posts: 32
Default Seeking advice on setting up a breakpoint detection

Greetings everyone,

as a beginner with the Illumina GA IIx I am currently sitting on a mate-pair run I'd love to screen vor chromosomal breakpoints, preferrably with BreakDancer.

But for some reason, I feel a bit stuck and could use a big push into the right direction. Is there any comprehensive resource on the net that demonstrates how to get from GA IIx Basecalls from a mate-pair-library (Pipeline 1.6, managed to align to hg19 with GERALD) to a structural variation analysis?

Thanks for letting me steal your time. :/

Best regards,
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