Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Did low input library and traditional library generated RNAseq data can be compared? bioinforD Bioinformatics 6 11-25-2013 07:57 AM

Thread Tools
Old 11-19-2020, 12:08 PM   #1
Junior Member
Location: Porton Down

Join Date: Apr 2016
Posts: 2
Default RNAseq library for polysome profiling (monosome and polysome-associated RNA)

Hi there,

I am interested in performing polysome profiling with downstream application for RNAseq of monosome and polysome-associated RNAs.

Has anyone had much experience with choice of library preparations for monosome and polysome-associated RNAs?

ribosomal RNA surely is to be more abundant in these samples compared to total RNA extractions? This would lead me towards preferring ribosome-depletion (e.g. riboZero) as opposed to polyA. However PolyA is better for intact fragments and we also intend to sequence total RNA where polyA would be preferred. If we want to compare sequencing between total RNA and mono/poly RNAs it would make sense to use the same library prep?

If you have any experience in choice of library prep (poly A vs ribo-depletion) for polysome-profiling RNAseq experiments please let me know which method you preferred and why

Many thanks,
JoeTaylor is offline   Reply With Quote

library prep, poly a, ribo zero, rnaseq, sequencing

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:31 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO