Hello experts,
I ran bowtie2 mapping with "local" setting on my illumina datasets a while ago and sam files were generated. Basically, I mapped reads onto assembled contigs. Unfortunately, sequence reads used for mapping are not available anymore so I need run bowtie2 again without "local" setting. Is there any way that I can do something with the sam files generated before? Any comments would be appreciated.
I ran bowtie2 mapping with "local" setting on my illumina datasets a while ago and sam files were generated. Basically, I mapped reads onto assembled contigs. Unfortunately, sequence reads used for mapping are not available anymore so I need run bowtie2 again without "local" setting. Is there any way that I can do something with the sam files generated before? Any comments would be appreciated.
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