1/4th of microtitre plate enough to characterize transcriptome of non-model legume?

cerebralrust · 04-27-2012, 07:48 AM

Dear Members,

In your experience, do you think 1/4th of a microtitre plate is enough to characterize the genome of a non-model legume species?

The 454 sequencing performed in the above fashion only resulted in a few hundred thousand reads of a not-so great coverage.

I was under the impression that,to fully characterize a transcriptome, one must sequence several samples of many genotypes in illumina and 454, combine the data and assemble, annotate etc, to achieve a good quality transcriptome that may represent the species.

What do you think?

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04-27-2012, 07:48 AM	#1
cerebralrust Junior Member Location: Sweden Join Date: Jan 2012 Posts: 8	1/4th of microtitre plate enough to characterize transcriptome of non-model legume? Dear Members, In your experience, do you think 1/4th of a microtitre plate is enough to characterize the genome of a non-model legume species? The 454 sequencing performed in the above fashion only resulted in a few hundred thousand reads of a not-so great coverage. I was under the impression that,to fully characterize a transcriptome, one must sequence several samples of many genotypes in illumina and 454, combine the data and assemble, annotate etc, to achieve a good quality transcriptome that may represent the species. What do you think?