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Similar Threads
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| Thread | Thread Starter | Forum | Replies | Last Post |
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04-14-2011, 08:40 AM
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#1 |
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Member
Location: NC Join Date: Apr 2011
Posts: 17
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Separate forward and reverse coverage in Artemis
Hi all,
Do you know how to demonstrate the reverse strand in the <0 region when you input *.BAM file including all the mapped reads in both forward and reverse strand? 
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04-14-2011, 11:59 AM
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#2 |
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Nils Homer
Location: Boston, MA, USA Join Date: Nov 2008
Posts: 1,285
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This question is not making sense to me. Could you clarify?
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04-14-2011, 12:27 PM
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#3 | |
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Member
Location: NC Join Date: Apr 2011
Posts: 17
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Quote:
After you read a BAM file(a mapped RNA-seq reads file) using Atermis, you'll see the expressed coding regions covered by many overlaped reads. However, the mapped reads from both forward strand and reverse strand are piled up in the above zero region. I'm considering a way to differentiate these reads, such that the forward strand reads can be piled up in the above zero region while the reads from the reverse strand can be piled up in below zero region... 
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12-11-2011, 12:14 PM
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#4 |
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Junior Member
Location: Toronto, ON Join Date: Oct 2011
Posts: 1
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I'm having the same issue. Has anyone found a solution?
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12-12-2011, 04:20 AM
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#5 |
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Peter (Biopython etc)
Location: Dundee, Scotland, UK Join Date: Jul 2009
Posts: 1,543
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Have you explored the right click menu? Artemis/BamView has several ways of displaying BAM files and I think one of them is strand specific.
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