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Old 03-05-2009, 08:02 AM   #1
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Location: cambridge, ma

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Default (a) Background Read Distribution (b) Spike-ins

Posted these under introductions, hence they may have been overlooked
Questions for the community:
Are there established error or background read distributions that would allow me to attach a p-value to an observed read count?

We are planning to use spike-ins to use observed read-counts to estimate transcript copy number. Are any of you using spike-ins in your sequencing experiments? If so, how are you estimating amount of spike-in to add?

ANJAN PURKAYASTHA is offline   Reply With Quote

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