SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
Periodic variation in nucleotide distribution along the read & other strange things lionelguy Illumina/Solexa 16 08-03-2011 10:04 AM
Read distribution at high sequence depth ForeignMan General 10 05-26-2011 04:50 AM
Periodical illumina read length distribution after trimming of low-quality bases luxmare General 4 12-20-2010 04:18 PM
RNA-seq read distribution wenhuang RNA Sequencing 9 11-08-2010 06:00 PM
rna-seq read distribution wenhuang Bioinformatics 1 06-17-2010 10:07 AM

Reply
 
Thread Tools
Old 03-05-2009, 08:02 AM   #1
ANJAN PURKAYASTHA
Junior Member
 
Location: cambridge, ma

Join Date: Mar 2009
Posts: 3
Default (a) Background Read Distribution (b) Spike-ins

Posted these under introductions, hence they may have been overlooked
Questions for the community:
Are there established error or background read distributions that would allow me to attach a p-value to an observed read count?

We are planning to use spike-ins to use observed read-counts to estimate transcript copy number. Are any of you using spike-ins in your sequencing experiments? If so, how are you estimating amount of spike-in to add?

TIA,
Anjan
ANJAN PURKAYASTHA is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:28 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO