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03-05-2009, 08:02 AM
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Junior Member
Location: cambridge, ma Join Date: Mar 2009
Posts: 3
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(a) Background Read Distribution (b) Spike-ins
Posted these under introductions, hence they may have been overlooked
Questions for the community: Are there established error or background read distributions that would allow me to attach a p-value to an observed read count? We are planning to use spike-ins to use observed read-counts to estimate transcript copy number. Are any of you using spike-ins in your sequencing experiments? If so, how are you estimating amount of spike-in to add? TIA, Anjan 
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