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Old 12-23-2011, 08:40 PM   #1
Raa
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Location: India

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Default Can we extract f3 reads while f5 reads are being sequenced in paired end

Hi,
I am naive to NGS. I want to know if we can take data for F3 reads (from p1 adaptor) while F5 reads are being sequenced on the machine. I have less time to analyse the data to submit a grant.

I want to do paired end sequencing but the time is less for submitting. I want to extract f1 read data for analysis during the run.
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Old 12-25-2011, 01:42 PM   #2
pmiguel
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Unless things have changed recently, the first set of reads are available before the second set of reads complete. So, depending on your downstream analysis methodology you should, in principle, be able to begin mapping before your second set of reads finishing.

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Phillip
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Old 12-25-2011, 09:46 PM   #3
Raa
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Thank you Philip for your help.
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