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Thread | Thread Starter | Forum | Replies | Last Post |
50 bp paired end reads vs. 100 bp single end reads | efoss | Bioinformatics | 12 | 01-15-2014 09:05 PM |
Extract paired end reads from sff file. | ojy | Bioinformatics | 4 | 12-13-2012 05:07 AM |
Can Cuffdiff treat paired-end and single-end reads at the same time? | zun | RNA Sequencing | 3 | 06-12-2012 06:37 PM |
paired-end reads mapped to genome.. gene with only one direction of paired-end reads? | danwiththeplan | Bioinformatics | 2 | 09-22-2011 03:06 AM |
How to extract paired-end reads from .sff 454? | pmiguel | Bioinformatics | 8 | 02-22-2010 09:17 AM |
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#1 |
Junior Member
Location: India Join Date: Nov 2011
Posts: 6
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Hi,
I am naive to NGS. I want to know if we can take data for F3 reads (from p1 adaptor) while F5 reads are being sequenced on the machine. I have less time to analyse the data to submit a grant. I want to do paired end sequencing but the time is less for submitting. I want to extract f1 read data for analysis during the run. |
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#2 |
Senior Member
Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008
Posts: 2,317
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Unless things have changed recently, the first set of reads are available before the second set of reads complete. So, depending on your downstream analysis methodology you should, in principle, be able to begin mapping before your second set of reads finishing.
-- Phillip |
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#3 |
Junior Member
Location: India Join Date: Nov 2011
Posts: 6
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Thank you Philip for your help.
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