I have uploaded the CSHL Long RNA-seq fastq dataset from the UCSC genome browser to galaxy and attempted to map the reads using bowtie, bwa and tophat. None of them are able to align more than a super small fraction of the reads. I have used each of these programs to align exactly the same types fastq data before, and it has worked perfectly. Does anyone have any suggestions as to what the problem could be?
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by seqadmin
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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05-06-2024, 07:48 AM -
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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